‘Genome gating’; polarized intranuclear trafficking of influenza virus RNPs

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'Genome gating'; polarized intranuclear trafficking of influenza virus RNPs.

Many viruses exploit cellular polarity to constrain the assembly and release of progeny virions to a desired surface. Influenza virus particles are released only from the apical surface of epithelial cells and this polarization is partly owing to specific targeting of the viral membrane proteins to the apical plasma membrane. The RNA genome of the virus is transcribed and replicated in the nucl...

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Intranuclear trafficking

Nucleic acids and regulatory proteins involved in gene expression and DNA replication are localized in specialized subnuclear domains that can be visualized by microscopy. However, the rules that govern the compartmentalization of the nucleus into these discrete domains have been elusive. From an historical perspective, the concept of nuclear subdivisions as functional compartments initially fo...

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Cytoplasmic and nuclear input virus RNPs in influenza virus-infected cells.

Chicken fibroblasts and MDCK cells were infected with influenza virus labelled with either 3H-uridine or 14C-amino acids, and the location in infected cells and properties of input virus-labelled structures were studied. Input virus RNA and protein were found in the cytoplasm of nuclei 1 h p.i. A part of the intranuclear parental structures was associated with chromatin while the other part cou...

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New Anti-Influenza Agents: Targeting the Virus Entry and Genome Transcription

Introduction: The emergence and spread of the pandemic H1N1 influenza virus in 2009 indicates a limitation in the strategy to control the infection, despite a long-established vaccination programme and approved antivirals. Production the proper vaccine against influenza is difficult due to the genetic recombination of virus in the event of pandemic and co-circulation of drug-resistance variants...

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ژورنال

عنوان ژورنال: Biology Letters

سال: 2005

ISSN: 1744-9561,1744-957X

DOI: 10.1098/rsbl.2004.0253